rabbit polyclonal pea15 antibody  (Proteintech)

Journal: Journal of Cancer

Article Title: The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis

doi: 10.7150/jca.32886

Figure Lengend Snippet: Correlation of PEA15 expression with patient's clinical and pathological characteristics

Article Snippet: Rabbit polyclonal PEA15 antibody (1:200, Proteintech, USA), PEA-15 with phosphorylated Ser104 (PEA-15-pSer104), or PEA-15with phosphorylated Ser116 (PEA-15-pSer116)(Santa Cruz Biotechnology, Inc., Shanghai, China) were used.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis

doi: 10.7150/jca.32886

Figure Lengend Snippet: PEA15 expression in OC tissue samples and immunohistochemical staining of two phosphorylation sites S116 and S104 of PEA15in normal and tumor tissues. A-D: Representative images of PEA15 expression in OC are shown at 200x magnification. A: OC, scored as (-); B: OC, scored as (+); C: OC, scored as (++); D: OC, scored as (+++). E: Normal ovarian tissue with negative PEA15-Ser104 staining and tumor tissue with positive PEA15-Ser104 staining. F: Normal ovarian tissue with negative PEA15-Ser116 staining and tumor tissue with positive PEA15-Ser116 staining.

Article Snippet: Rabbit polyclonal PEA15 antibody (1:200, Proteintech, USA), PEA-15 with phosphorylated Ser104 (PEA-15-pSer104), or PEA-15with phosphorylated Ser116 (PEA-15-pSer116)(Santa Cruz Biotechnology, Inc., Shanghai, China) were used.

Techniques: Expressing, Immunohistochemical staining, Staining, Phospho-proteomics

Journal: Journal of Cancer

Article Title: The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis

doi: 10.7150/jca.32886

Figure Lengend Snippet: Kaplan-Meier analysis of overall survival in OC patients based on K-M plotter dataset. A: PEA15 expression is negatively correlated with overall survival (OS) and progression free survival (PFS) in OC patients; B-D: Correlation between PEA15 expression and overall survival is independent of clinical stage ( B ), type ( C ) and Grade ( D ). P -values were calculated by log-rank test.

Article Snippet: Rabbit polyclonal PEA15 antibody (1:200, Proteintech, USA), PEA-15 with phosphorylated Ser104 (PEA-15-pSer104), or PEA-15with phosphorylated Ser116 (PEA-15-pSer116)(Santa Cruz Biotechnology, Inc., Shanghai, China) were used.

Techniques: Expressing

Journal: Journal of Cancer

Article Title: The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis

doi: 10.7150/jca.32886

Figure Lengend Snippet: Silencing of PEA15 suppresses ovarian cancer cell proliferation in vitro and tumor growth in vivo . A: RT-PCR and western blotting experiments showing the PEA15 expression at mRNA and protein level in 5 OC cell lines. B: PEA15 knockdown efficiency was confirmed by RT-PCR in OVCAR8 and 3AO cells. C: The cell proliferation of Nc and sh-groups in OVCAR8 and 3AO cells were evaluated by CCK8 assay at 0, 24, 48, 72, 96h. The results showed that knockdown of PEA15 significantly inhibited the proliferation of OVCAR8 and 3AO cells in vitro (P<0.01). D: Plate colony formation assay of OVCAR8/PEA15-sh and 3AO/PEA15-sh and Nc cells on regular culture plates after 14 days of culture. Relative colony numbers of PEA15-sh cells were significantly lower than that of Nc cells. E: Photographs of tumors from mice inoculated with OVCAR8/Nc and OVCAR8/sh3 cells. F: Tumor weights of Nc and sh3 groups shown in figure F, n = 5

Article Snippet: Rabbit polyclonal PEA15 antibody (1:200, Proteintech, USA), PEA-15 with phosphorylated Ser104 (PEA-15-pSer104), or PEA-15with phosphorylated Ser116 (PEA-15-pSer116)(Santa Cruz Biotechnology, Inc., Shanghai, China) were used.

Techniques: In Vitro, In Vivo, Reverse Transcription Polymerase Chain Reaction, Western Blot, Expressing, Knockdown, CCK-8 Assay, Colony Assay

Journal: Journal of Cancer

Article Title: The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis

doi: 10.7150/jca.32886

Figure Lengend Snippet: Effect of PEA15 on cell apoptosis in OC cells. A-B: Downregulation of PEA15 significantly increased apoptosis in OVCAR8 and 3AO cells, the statistical results are shown on the left (* P < 0.05, ** P < 0.01). C-D: The expression of Bcl2, Bax and cleaved caspase3 were determined by RT-PCR and western blotting analysis in OVCAR8 and 3AO cells.

Article Snippet: Rabbit polyclonal PEA15 antibody (1:200, Proteintech, USA), PEA-15 with phosphorylated Ser104 (PEA-15-pSer104), or PEA-15with phosphorylated Ser116 (PEA-15-pSer116)(Santa Cruz Biotechnology, Inc., Shanghai, China) were used.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Journal of Cancer

Article Title: The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis

doi: 10.7150/jca.32886

Figure Lengend Snippet: PEA15 is negatively regulated by miR212 and silencing of PEA15 abolishes the effects of miR212 in OC cell lines. A: A nalysis of TargetScan, miRwalk, picTar and miRBse datasets identified 3 miRNAs as potential target of PEA15. B-D: Effect of 3 miRNAs on the luciferase activity of WT PEA-15 3′UTR in OVCAR8 cells by luciferase reporter assay. Blank PEA-15 negative vector, Mock 3 miRNAs plus PEA-15 negative vector. Error bars mean ± SEM. E: The expression of miR212 is downregulated in ovarian tumor tissues compared with normal tissues. F: The expression of miR212 in 5 ovarian cancer cells. G: The protein expression of PEA-15 is down-regulated when miR-212 is overexpressed. H: Effect of reintroduction of PEA-15 on miR-212-induced cell proliferation by CCK-8 assay ( P < 0.01 with two-way ANOVA). I: Effect of reintroduction of PEA-15 on miR-212-induced cell apoptosis by Flow analysis ( P < 0.01 for both groups).

Article Snippet: Rabbit polyclonal PEA15 antibody (1:200, Proteintech, USA), PEA-15 with phosphorylated Ser104 (PEA-15-pSer104), or PEA-15with phosphorylated Ser116 (PEA-15-pSer116)(Santa Cruz Biotechnology, Inc., Shanghai, China) were used.

Techniques: Luciferase, Activity Assay, Reporter Assay, Plasmid Preparation, Expressing, CCK-8 Assay

Journal: Cell Systems

Article Title: Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling

doi: 10.1016/j.cels.2016.11.013

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-PEA15 , Cell Signaling Technology , Cat#2780; RRID: AB_2268149.

Techniques: Transduction, Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Software, Inhibition, Modification

Journal: Molecular & Cellular Proteomics

Article Title: Brief Alteration of NMDA or GABAA Receptor-mediated Neurotransmission Has Long Term Effects on the Developing Cerebral Cortex

doi: 10.1074/mcp.m800030-mcp200

Figure Lengend Snippet: FIG. 2. Protein expression groups throughout brain development and in adult age. A, protein spots can be classified in the following expression groups (EGs): (i) stable EG: protein spot abundance is largely unchanged at all evaluated ages; (ii) early EG: protein spots can be visualized early in development but diminish or disappear with age; (iii) late EG: proteins appear at later ages; and (iv) transient EG: protein spots can be visualized only within a specific period. B, this classification that holds true for one isoprotein is not necessarily applicable for all spots assigned to one protein (isoproteins). On a 2-DE gel, one protein may be represented by one spot or constitute a pattern of multiple spots (isospots) caused e.g. by co- and/or post-translational modifications of the primary protein product or by protein processing. CRMP2 demonstrated a differential regulation throughout development when investigated by Western blotting of 2-DE gels with a CRMP2-specific antibody. This approach revealed 27 isospots at P7, the abundance of most isospots was strongly reduced in older mice, and 13 could not be visualized anymore at P35 (early EG). However, four and five isospots that could not be detected at P7 appeared at P14 and P35, respectively (late expression group). C, several proteins such as PEA15 showed an increase in the number of isospots throughout development: two PEA15 isospots were detectable at P7, their abundance increased with age, and at P14 a further isospot appeared.

Article Snippet: Membranes were incubated with rabbit anti-CRMP2 polyclonal antibody (Chemicon, Temecula, CA) at a dilution of 1:500, rabbit anti-CRMP4 polyclonal antibody (Chemicon) at a dilution of 1:1000, or rabbit anti-PEA15 polyclonal antibody (Cell Signaling Technology, Beverly, MA) at a dilution of 1:1000, respectively.

Techniques: Expressing, Western Blot

Journal: Molecular & Cellular Proteomics

Article Title: Brief Alteration of NMDA or GABAA Receptor-mediated Neurotransmission Has Long Term Effects on the Developing Cerebral Cortex

doi: 10.1074/mcp.m800030-mcp200

Figure Lengend Snippet: FIG. 4. PEA15 isoproteins. Protein spots on 2-DE gels from 2-week-old C57BL/6 mice identified as PEA15 by MS are marked by circles and identified by letters (refer to Table IV for MS results).

Article Snippet: Membranes were incubated with rabbit anti-CRMP2 polyclonal antibody (Chemicon, Temecula, CA) at a dilution of 1:500, rabbit anti-CRMP4 polyclonal antibody (Chemicon) at a dilution of 1:1000, or rabbit anti-PEA15 polyclonal antibody (Cell Signaling Technology, Beverly, MA) at a dilution of 1:1000, respectively.

Techniques: